[LAB] Assay of Ascorbic acid with Calculations


Aim:

To carry out assay of Ascorbic Acid.

Chemical requirements:

  • Ascorbic acid
  • Dil Sulphuric acid
  • Starch solution (Indicator)
  • 0.1 N Iodine Solution

Apparatus

  1. Burette
  2. Conical flask
  3. Beaker
  4. Glassrod
  5. Measuring cylinder

Procedure

  1. Weigh about 0.1 gram of ascorbic acid accurately.
  2. Dissolve in a mixture of 100 ml of freshly boiled and cooled water and 25 ml of dil. sulphuric acid
  3. Tirate immediately with 0.1 N Iodine solution using starch as indicator as the endpoint is clear blue colour

Each ml of 0.1N Iodine solution ~ 0.008806 grams of Ascorbic acid (C6H8O4)

To view calculations and viva part of Assay of Ascorbic acid click here

[button url=”http://pharmawiki.in/wp-content/uploads/2014/01/Assay-of-Ascorbic-Acid-with-Calculations.pdf” style=”glass” background=”#1782f9″ color=”#ffffff” size=”4″ center=”yes” icon=”icon: download”]Download PDF here[/button]

To view calculations part of Preparation and Standardisation of 0.1N Iodine Solution click here