Aim:

To carry out assay of Ascorbic Acid.

Chemical requirements:

• Ascorbic acid
• Dil Sulphuric acid
• Starch solution (Indicator)
• 0.1 N Iodine Solution

Apparatus

1. Burette
2. Conical flask
3. Beaker
4. Glassrod
5. Measuring cylinder

Procedure

1. Weigh about 0.1 gram of ascorbic acid accurately.
2. Dissolve in a mixture of 100 ml of freshly boiled and cooled water and 25 ml of dil. sulphuric acid
3. Tirate immediately with 0.1 N Iodine solution using starch as indicator as the endpoint is clear blue colour

Each ml of 0.1N Iodine solution ~ 0.008806 grams of Ascorbic acid (C₆H₈O₄)

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To view calculations part of Preparation and Standardisation of 0.1N Iodine Solution click here