[LAB] Assay of Ascorbic acid with Calculations


To carry out assay of Ascorbic Acid.

Chemical requirements:

  • Ascorbic acid
  • Dil Sulphuric acid
  • Starch solution (Indicator)
  • 0.1 N Iodine Solution


  1. Burette
  2. Conical flask
  3. Beaker
  4. Glassrod
  5. Measuring cylinder


  1. Weigh about 0.1 gram of ascorbic acid accurately.
  2. Dissolve in a mixture of 100 ml of freshly boiled and cooled water and 25 ml of dil. sulphuric acid
  3. Tirate immediately with 0.1 N Iodine solution using starch as indicator as the endpoint is clear blue colour

Each ml of 0.1N Iodine solution ~ 0.008806 grams of Ascorbic acid (C6H8O4)


To view calculations and viva part of Assay of Ascorbic acid click here

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To view calculations part of Preparation and Standardisation of 0.1N Iodine Solution click here

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